Evaluation the Effect of Natural Compounds: Vitamin C, Green Tea, and their Combination on Progression of Mg-63 Osteosarcoma Cell Line Cells. (An In Vitro Study)

BACKGROUND: Osteosarcoma (OS) is considered extremely rare type of bone tumor although it the most common malignant in children with less occurrence elderly patients. Herbal plants and phytoconstituents are recently used treatment OS to avoid side effects chemotherapeutic drugs. AIM: The aims present study investigate effect natural compound Vitamin C, green tea, their combination on cell line (Mg-63 cells) after 72 h. MATERIAL AND METHODS: Mg-63 cells were obtained from Nawah scientific divided four groups: Control untreated cells, C treated group, tea group (compounds group). viability was examined by sulforhodamine B (SRB) assay. Antioxidant 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay performed antioxidant property combination. Flow cytometer analysis applied demonstrate cycle apoptosis. Wound width migration calculated wound healing RESULTS: SRB cytotoxic revealed that have a MG-63 has more than other two groups. DPPH showed agent cells. cytometry all-treated different groups arrested cycle. induced apoptosis necrosis. Migration inhibited CONCLUSION: also distribution Anti-oxidant test three drugs powerful anti-oxidant capacity At least our effective.